线性PEI转染试剂(25K)
英文名 Polyethylenimine Linear(PEI) MW25000
货号 JX23966
保存条件 室温
概述
线性PEI转染试剂是一种阳离子聚合物,分子量25000,它能与核酸形成复合物,并使该复合物进入哺乳动物细胞。线性PEI转染试剂广泛适用于常见细胞系,如 HEK-293、HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 和 Sf9 等。该试剂即使在有血清存在的情况下,它仍然能高效的将核酸导入细胞。
我公司提供的线性PEI转染试剂具有如下优点:优越的转染效率 , 重组蛋白的高表达水平,与含血清的培养基相兼容, 低细胞毒性,易于操作。
Transfection Reagent Preparation and Storage Recommendations
These guidelines are our recommendations based off research, testing, and customer feedback. There are alternative methods available, but we suggest following these recommendations for easy adoption and consistently high performance. These methods are adapted from Cold Spring Harbor Protocols, an excellent secondary resource for transient transfection literature.
Reagent Preparation (1 mg/mL)
Materials:
6.Sterile HDPE or polypropylene storage vials for reagent aliquots.
Equipment
8.Aliquot as desired and store at -20℃.
Reagent Storage
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