线性PEI转染试剂（速溶型）MW40000
英文名 Polyethylenimine Linear(PEI) MW40000（rapid lysis）
货号 JX24765
保存条件 室温
线性PEI转染试剂是一种阳离子聚合物，分子量40000，它能与核酸形成复合物，并使该复合物进入哺乳动物细胞。线性PEI转染试剂广泛适用于常见细胞系，如 HEK-293、HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 和 Sf9 等。该试剂即使在有血清存在的情况下，它仍然能高效的将核酸导入细胞。我公司提供的线性PEI转染试剂具有如下优点：优越的转染效率 , 重组蛋白的高表达水平,与含血清的培养基相兼容, 低细胞毒性,易于操作。
本产品为速溶型，溶解迅速，配制方便。
说明书
Transfection Reagent Preparation and Storage Recommendations
Transfection Reagent Preparation (1 mg/mL)
Materials:
1.1g PEI 25K (kingmorn Catalog# KE1075).
2.1L Milli-Q® water, water for injection (WFI), or comparable biological-grade water
3.12 M Hydrochloric Acid (HCl)
4.10 M Sodium Hydroxide (NaOH)
5.Disposable 0.1-0.2um PES vacuum sterile-filter.
6.Sterile HDPE or polypropylene storage vials for reagent aliquots.
Equipment
1.1L glass beaker
2.1L glass graduated cylinder
3.Calibrated pH meter
4.2 x disposable 1mL plastic pipettes
5.Stir plate
6.PTFE coated stir bar
7.Vacuum pump
Method:
1.Dispense 1g of PEI 25K into beaker and suspend in 900 mL of water.
2.Add stir bar and set stirring to produce a small vortex.
3.Add hydrochloric acid dropwise until pH is < 2.0.
4.Cover top of beaker and stir for up to 3 hours until powder fully dissolves.
5.Add sodium hydroxide dropwise until pH is 6.9-7.1.
6.Transfer solution to graduated cylinder and add water until the total volume is 1L.
7.Sterile-filter through vacuum membrane.
8.Aliquot as desired and store at -20℃.
Reagent Storage
Frozen aliquots can be stored at -20C for up to one year. Aliquots can be thawed and kept at 4C for up to two weeks, but should never be re-frozen.
References
1. Polyethylenimine (PEI), linear (1 mg/mL). Cold Spring Harbor Protocols 2008, pdb.rec11323– pdb.rec11323 (2008). doi:10.1101/pdb.rec11323
