GxBeadsTM 磁珠 Protein A Magnetic Beads for IP/CoIP/ChIP
货号:JXB003
规格:1mL /5mL /10mL
产品描述
Background: Protein A/G Magnetic Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein A/G is covalently coupled to a magnetic particle. Protein A/G exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies. Beads can be separated from solution using our 6-Tube Magnetic Separation Rack or 12-Tube Magnetic Separation Rack which concentrates the beads to the side of the tube instead of the bottom. This eliminates centrifugation steps, minimizes sample loss, increases washing efficiency, and saves time. The 1mL and 5 mL size is enough material for 25 and 125 immunoprecipitations, respectively, when following our recommended protocol.
Specificity:Protein A/G Magnetic Beads is suitable for immunoprecipitation of mouse IgG1, IgG2a, IgG2b, IgG3, rat IgG1, IgG2a, IgG2b and IgG2c, rabbit IgG and goat IgG1, IgG2, IgG3 and IgG4.
Tested applications: Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP),
Chromatin Immunoprecipitation (ChIP),
Suitable for use at 25-40 µL beads slurry per IP reaction. Optimal amount should be determined by the end user.
Form: Supplied in PBS (pH 7.4), and 0.02% sodium azide.
Bead Diameter: 1 µm
Beads Concentration: 10 mg settled beads/ 1 mL beads slurry
Binding Capacity: > 600 µg of antibody (rabbit IgG or mouse IgG) can be bound per ml of beads slurry.
Storage instruction:
Store at 4°C. This product is stable for 12 months. Do not freeze, dry or centrifuge beads. This may cause irreversible aggregation and decreased binding capacity.
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