GF®594 - Phalloidin 标记鬼笔环肽 - 300T

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产品名称: GF®594 - Phalloidin 标记鬼笔环肽 - 300T

产品型号: JXF40171 - 300T

产品展商: 晶欣生物

产品文档:

GF®594 Phalloidin标记鬼笔环肽说明书

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GF®594 Phalloidin标记鬼笔环肽

货号：JXF40171-300T

规格：300T

价格：1200元

NTRODUCTION

Phalloidin is a bicyclic peptide that belongs to a family of toxins isolated from the deadly Amanita

phalloides mushroom. Fluorescent phalloid ins bind F-actin with nanomolar affinity and are water soluble,

thus providing convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections,

cell cultures, or cell-free experiments. halloid in contains an unusual thioether bridge between cysteine and

tryptophan residues that forms an inner ring structure. At elevated pH, this thioether is cleaved and the toxin loses

its affinity for actin. Fluorescently labeled phalloid ins stain F-actin at nanomolarconcentrations. Labeled phalloidins

have similar affinity for both large and small filaments, binding in a stoichiometric ratio of about one phalloid in

molecule per actin subunit in muscle and non-muscle cells from various species of plants and animals.

Different from antibodies, the binding affinity of phalloid in does not change significantly with actin among different

species.Non-specific staining is negligible, and the contrast between stained and unstained areas is extremely large.

Phalloid in shifts the monomer/polymer equilibrium toward the polymer, lowering the critical concentration for

polymerization up to 30-fold. Phallotoxins also stabilize F-actin, inhibiting depolymerization by cytochalasin, potassium iodide and elevated temperatures. Because the phalloidin conjugates are small, with an approximate diameter of 12-15Å

and molecular weight of <2000 Daltons, a variety of actin-binding proteins including myosin, tropomyosin and troponin

can still bind to actin after treatment with phalloidin. Even more significantly, phalloid in-labeled actin filaments remain

functional; labeled glycerinated muscle fibers still contract, and labeled actin filaments still move on solid-phase myosin

substrates. Fluorescent phalloid in can also be used to quantify the amount of F-actin in cells.