· CAS号 9004-67-5
biological source :
wood (pulp cellulose)
form :
powder
viscosity :
3,500-5,600 cP, 2 % in water(20 °C)
Methyl cellulose is a water-soluble, non-ionic polymer with hydrophilic glucosidic bonds, derived from the polysaccharide cellulose.
Methyl cellulose has been used: • in colony forming assays • for sperm motility assessment • for the generation of spheroids • in preparation of agar beads
MM0512 is recommended over glycerol for use in a cryopreservation medium; a higher percentage of viability was noted for each organism tested in a 1% methylcellulose solution compared to a 15% glycerol solution. The Sensitivity and resistance of the preserved organisms to various antibiotics did not change.4 Methylcellulose of 4000 cps (MM0512) was used in a semi-solid culture medium; plating cells in 1.2% methylcellulose with 10% fetal calf serum were plated over a layer of 0.9% agar.5,6 Similar applications involved plating cells in an 0.8% solution.7,8 Human neuroblastoma cells were cloned and cultured successfully in a 1% methylcellulose medium.9 A standard protocol for plating in Methocel (MM0512) using 1.6% final concentration in medium has been published
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