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脱氧核糖核酸酶 I 来源于牛胰腺

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产品名称: 脱氧核糖核酸酶 I 来源于牛胰腺
产品型号: MDN25-100mg
产品展商: MBCHEM
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脱氧核糖核酸酶 I 来源于牛胰腺

Deoxyribonuclease I from bovine pancreas



别名DNase I, Deoxyribonuclease, 脱氧核糖核酸 5′-寡核苷酸-水解酶

lyophilized powder, Protein ≥85 %, ≥400 Kunitz units/mg protein


CAS 9003-98-9

orm  :lyophilized powder

mol wt  :~31 kDa

composition:Protein, ≥85%

solubility :0.15 M NaCl: soluble 5.0 mg/mL, hazy

foreign activity  :RNase ≤0.02%


适用:

Application

• DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA.
• DNAse can be used for Degradation of DNA templates in transcription reactions
• DNAse has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture. 
• Deoxyribonuclease I has also been used along with trypsin for the preparation of single cell suspension from rat testes. 
• Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase.
• Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
• This DNase can be used for the removal of DNA from protein samples.
• Preventing the loss of cells from undesired clumping during tissue dissociation: parts of the cells which are lysed, resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, thereby.

用于从蛋白质样品中除去 DNA


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