DTT (Dithiothreitol) 二硫苏糖醇

≥99.0%, Ultra Pure Grade

货号：MD0281-10g   300.00

货号：MD0281-100G  2000.00

别名: DL-1,4-Dithiothreitol , DTT , (R*,R*)-1,4-Dimercapto-2,3-butandiol

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).